(Heb. pered), so called from the quick step of the animal or its power of carrying loads. It is not probable that the Hebrews bred mules, as this was strictly forbidden in the law (Leviticus 19:19), although their use was not forbidden. We find them in common use even by kings and nobles (2 Samuel 18:9; 1 Kings 1:33; 2Kings 5:17; Psalms 32:9). They are not mentioned, however, till the time of David, for the word rendered "mules" (RSV correctly, "hot springs") in Genesis 36:24 (yemim) properly denotes the warm springs of Callirhoe, on the eastern shore of the Dead Sea. In David's reign they became very common (2 Samuel 13:29; 1 Kings 10:25).
Mules are not mentioned in the New Testament. Perhaps they had by that time ceased to be used in Palestine.
These dictionary topics are from M.G. Easton M.A., D.D., Illustrated Bible Dictionary, Third Edition, published by Thomas Nelson, 1897. Public Domain, copy freely.
Easton, Matthew George. "Entry for 'Mule'". "Easton's Bible Dictionary".